Polyacrylamide Gel Electrophoresis:Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Invitrogen Cat# 15527013 Gold (III) chloride trihydrate Sigma-Aldrich Cat# 520918; CAS: 16961-25-4 Iodoacetamide Sigma-Aldrich Cat# A3221; CAS: 144-48-9 Keratinocyte serum free medium (KSFM) Gibco Cat# 17005042 MBA Sigma-Aldrich Cat# 706329; CAS: 1074-36-8 Methanol Supelco Cat# 1.06009; CAS: 67-56-1 MPY Sigma-Aldrich Cat# 148202; CAS: 4556-23-4 Non-fat powdered milk Merck Cat# 1.15363; CAS: 999999-99-4 PBS tablets Gibco Cat# 18912014 Penicillin-Streptomycin Gibco Cat# 15140122 Precision Plus ProteinTM WesternCTM Blotting Standards Bio-Rad Cat# 1610376 Protease inhibitors Sigma-Aldrich Cat# P8340 Resolving Gel Buffer for PAGE Bio-Rad Cat# 1610798 RPMI 1640 Medium Gibco Cat# 11875093 SDS Bio-Rad Cat# 1610302; CAS: 151-21-3 Sodium azide Sigma-Aldrich Cat# 71289; CAS: 26628-22-8 Stacking Gel Buffer for PAGE Bio-Rad Cat# 1610799 TEMED Bio-Rad Cat# 1610801; CAS: 110-18-9 TFMBA TCI America Cat# T25425G; CAS: 5211-44-9 Trifluoroacetic acid Thermo Scientific Cat# 85183; CAS: 76-05-1 Tris Roche Cat# 10153265103; CAS: 77-86-1 Tri-Sodium citrate Ajax Finechem Cat# AJA467-500G; CAS: 68-04-2 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 Trypsin New England Biolabs (NEB) Cat# P8101S Tween 20 Sigma-Aldrich Cat# P1379; CAS: 9005-64-5 Western Blotting Detection Reagent Cytiva Cat# RPN2235 Critical commercial assays Human THBS1 DuoSet ELISA R&D Systems Cat# DY3074 Human NID1 DuoSet ELISA R&D Systems Cat# DY2570 Human PTX3 DuoSet ELISA R&D Systems Cat# DY1826 Human VCAN ELISA Kit Novus Biologicals Cat# NBP2-75353 Amicon® Ultra-4 10 kDa Millipore Cat# UFC8100 Micro BCATM Protein Assay Kit Thermo Scientific Cat# 23235 SYLGARDTM 184 Silicone Elastomer Kit Dow Cat# 761036 qEVoriginal 70nm SEC columns IZON Science Cat# ICO-70 Deposited data Mass spectrometry proteomics data This paper ProteomeXchange Consortium: PXD073635 Experimental models: Cell lines HBECs, 30KT Larsen et al.70,71 N/A 30KTp53/KRAS Larsen et al.70,71 N/A HOSE 6-3 Garvan Research Institute RRID: CVCL_7673 HOSE 17-1 Garvan Research Institute RRID: CVCL_7672 .. HCC4006 ATCC CRL-2871; RRID: CVCL_1269
Western Blot:Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Invitrogen Cat# 15527013 Gold (III) chloride trihydrate Sigma-Aldrich Cat# 520918; CAS: 16961-25-4 Iodoacetamide Sigma-Aldrich Cat# A3221; CAS: 144-48-9 Keratinocyte serum free medium (KSFM) Gibco Cat# 17005042 MBA Sigma-Aldrich Cat# 706329; CAS: 1074-36-8 Methanol Supelco Cat# 1.06009; CAS: 67-56-1 MPY Sigma-Aldrich Cat# 148202; CAS: 4556-23-4 Non-fat powdered milk Merck Cat# 1.15363; CAS: 999999-99-4 PBS tablets Gibco Cat# 18912014 Penicillin-Streptomycin Gibco Cat# 15140122 Precision Plus ProteinTM WesternCTM Blotting Standards Bio-Rad Cat# 1610376 Protease inhibitors Sigma-Aldrich Cat# P8340 Resolving Gel Buffer for PAGE Bio-Rad Cat# 1610798 RPMI 1640 Medium Gibco Cat# 11875093 SDS Bio-Rad Cat# 1610302; CAS: 151-21-3 Sodium azide Sigma-Aldrich Cat# 71289; CAS: 26628-22-8 Stacking Gel Buffer for PAGE Bio-Rad Cat# 1610799 TEMED Bio-Rad Cat# 1610801; CAS: 110-18-9 TFMBA TCI America Cat# T25425G; CAS: 5211-44-9 Trifluoroacetic acid Thermo Scientific Cat# 85183; CAS: 76-05-1 Tris Roche Cat# 10153265103; CAS: 77-86-1 Tri-Sodium citrate Ajax Finechem Cat# AJA467-500G; CAS: 68-04-2 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 Trypsin New England Biolabs (NEB) Cat# P8101S Tween 20 Sigma-Aldrich Cat# P1379; CAS: 9005-64-5 Western Blotting Detection Reagent Cytiva Cat# RPN2235 Critical commercial assays Human THBS1 DuoSet ELISA R&D Systems Cat# DY3074 Human NID1 DuoSet ELISA R&D Systems Cat# DY2570 Human PTX3 DuoSet ELISA R&D Systems Cat# DY1826 Human VCAN ELISA Kit Novus Biologicals Cat# NBP2-75353 Amicon® Ultra-4 10 kDa Millipore Cat# UFC8100 Micro BCATM Protein Assay Kit Thermo Scientific Cat# 23235 SYLGARDTM 184 Silicone Elastomer Kit Dow Cat# 761036 qEVoriginal 70nm SEC columns IZON Science Cat# ICO-70 Deposited data Mass spectrometry proteomics data This paper ProteomeXchange Consortium: PXD073635 Experimental models: Cell lines HBECs, 30KT Larsen et al.70,71 N/A 30KTp53/KRAS Larsen et al.70,71 N/A HOSE 6-3 Garvan Research Institute RRID: CVCL_7673 HOSE 17-1 Garvan Research Institute RRID: CVCL_7672 .. HCC4006 ATCC CRL-2871; RRID: CVCL_1269
Enzyme-linked Immunosorbent Assay:Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Invitrogen Cat# 15527013 Gold (III) chloride trihydrate Sigma-Aldrich Cat# 520918; CAS: 16961-25-4 Iodoacetamide Sigma-Aldrich Cat# A3221; CAS: 144-48-9 Keratinocyte serum free medium (KSFM) Gibco Cat# 17005042 MBA Sigma-Aldrich Cat# 706329; CAS: 1074-36-8 Methanol Supelco Cat# 1.06009; CAS: 67-56-1 MPY Sigma-Aldrich Cat# 148202; CAS: 4556-23-4 Non-fat powdered milk Merck Cat# 1.15363; CAS: 999999-99-4 PBS tablets Gibco Cat# 18912014 Penicillin-Streptomycin Gibco Cat# 15140122 Precision Plus ProteinTM WesternCTM Blotting Standards Bio-Rad Cat# 1610376 Protease inhibitors Sigma-Aldrich Cat# P8340 Resolving Gel Buffer for PAGE Bio-Rad Cat# 1610798 RPMI 1640 Medium Gibco Cat# 11875093 SDS Bio-Rad Cat# 1610302; CAS: 151-21-3 Sodium azide Sigma-Aldrich Cat# 71289; CAS: 26628-22-8 Stacking Gel Buffer for PAGE Bio-Rad Cat# 1610799 TEMED Bio-Rad Cat# 1610801; CAS: 110-18-9 TFMBA TCI America Cat# T25425G; CAS: 5211-44-9 Trifluoroacetic acid Thermo Scientific Cat# 85183; CAS: 76-05-1 Tris Roche Cat# 10153265103; CAS: 77-86-1 Tri-Sodium citrate Ajax Finechem Cat# AJA467-500G; CAS: 68-04-2 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 Trypsin New England Biolabs (NEB) Cat# P8101S Tween 20 Sigma-Aldrich Cat# P1379; CAS: 9005-64-5 Western Blotting Detection Reagent Cytiva Cat# RPN2235 Critical commercial assays Human THBS1 DuoSet ELISA R&D Systems Cat# DY3074 Human NID1 DuoSet ELISA R&D Systems Cat# DY2570 Human PTX3 DuoSet ELISA R&D Systems Cat# DY1826 Human VCAN ELISA Kit Novus Biologicals Cat# NBP2-75353 Amicon® Ultra-4 10 kDa Millipore Cat# UFC8100 Micro BCATM Protein Assay Kit Thermo Scientific Cat# 23235 SYLGARDTM 184 Silicone Elastomer Kit Dow Cat# 761036 qEVoriginal 70nm SEC columns IZON Science Cat# ICO-70 Deposited data Mass spectrometry proteomics data This paper ProteomeXchange Consortium: PXD073635 Experimental models: Cell lines HBECs, 30KT Larsen et al.70,71 N/A 30KTp53/KRAS Larsen et al.70,71 N/A HOSE 6-3 Garvan Research Institute RRID: CVCL_7673 HOSE 17-1 Garvan Research Institute RRID: CVCL_7672 .. HCC4006 ATCC CRL-2871; RRID: CVCL_1269
Mass Spectrometry:Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Invitrogen Cat# 15527013 Gold (III) chloride trihydrate Sigma-Aldrich Cat# 520918; CAS: 16961-25-4 Iodoacetamide Sigma-Aldrich Cat# A3221; CAS: 144-48-9 Keratinocyte serum free medium (KSFM) Gibco Cat# 17005042 MBA Sigma-Aldrich Cat# 706329; CAS: 1074-36-8 Methanol Supelco Cat# 1.06009; CAS: 67-56-1 MPY Sigma-Aldrich Cat# 148202; CAS: 4556-23-4 Non-fat powdered milk Merck Cat# 1.15363; CAS: 999999-99-4 PBS tablets Gibco Cat# 18912014 Penicillin-Streptomycin Gibco Cat# 15140122 Precision Plus ProteinTM WesternCTM Blotting Standards Bio-Rad Cat# 1610376 Protease inhibitors Sigma-Aldrich Cat# P8340 Resolving Gel Buffer for PAGE Bio-Rad Cat# 1610798 RPMI 1640 Medium Gibco Cat# 11875093 SDS Bio-Rad Cat# 1610302; CAS: 151-21-3 Sodium azide Sigma-Aldrich Cat# 71289; CAS: 26628-22-8 Stacking Gel Buffer for PAGE Bio-Rad Cat# 1610799 TEMED Bio-Rad Cat# 1610801; CAS: 110-18-9 TFMBA TCI America Cat# T25425G; CAS: 5211-44-9 Trifluoroacetic acid Thermo Scientific Cat# 85183; CAS: 76-05-1 Tris Roche Cat# 10153265103; CAS: 77-86-1 Tri-Sodium citrate Ajax Finechem Cat# AJA467-500G; CAS: 68-04-2 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 Trypsin New England Biolabs (NEB) Cat# P8101S Tween 20 Sigma-Aldrich Cat# P1379; CAS: 9005-64-5 Western Blotting Detection Reagent Cytiva Cat# RPN2235 Critical commercial assays Human THBS1 DuoSet ELISA R&D Systems Cat# DY3074 Human NID1 DuoSet ELISA R&D Systems Cat# DY2570 Human PTX3 DuoSet ELISA R&D Systems Cat# DY1826 Human VCAN ELISA Kit Novus Biologicals Cat# NBP2-75353 Amicon® Ultra-4 10 kDa Millipore Cat# UFC8100 Micro BCATM Protein Assay Kit Thermo Scientific Cat# 23235 SYLGARDTM 184 Silicone Elastomer Kit Dow Cat# 761036 qEVoriginal 70nm SEC columns IZON Science Cat# ICO-70 Deposited data Mass spectrometry proteomics data This paper ProteomeXchange Consortium: PXD073635 Experimental models: Cell lines HBECs, 30KT Larsen et al.70,71 N/A 30KTp53/KRAS Larsen et al.70,71 N/A HOSE 6-3 Garvan Research Institute RRID: CVCL_7673 HOSE 17-1 Garvan Research Institute RRID: CVCL_7672 .. HCC4006 ATCC CRL-2871; RRID: CVCL_1269
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